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Like different plant compartments, the seed harbors a microbiome.

The members of the seed microbiome are the primary to colonize a germinating seedling, and they may initiate the trajectory of microbiome meeting for the subsequent plant technology. Therefore, the members of the seed microbiome are necessary for the dynamics of plant microbiome meeting and the vertical transmission of potentially helpful symbionts.
However, it stays challenging to assess the microbiome at the individual seed level (and, subsequently, for the future individual plants) resulting from low endophytic microbial biomass, seed exudates that can select for specific members, and excessive plant and plastid contamination of resulting reads.
Here, we report a protocol for extracting microbial DNA from an individual seed (common bean, Phaseolus vulgaris) with minimal disruption of host tissue, which we expect to be generalizable to other medium- and large-seed plant species. We applied this protocol to determine the 16S ribosomal RNA (rRNA) V4 and rRNA internal transcribed spacer 2 amplicon composition and look at the variability of particular person seed harvested from replicate widespread bean plants grown below normal, managed conditions to maintain well being.

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